Journal: Journal of Ovarian Research

Article Title: The presence of living endometrial cells in ovarian endometriotic cyst fluid may contribute to the recurrence of endometriosis after surgical excision of endometriomas

doi: 10.1186/s13048-022-01018-9

Figure Lengend Snippet: Presence of living endometrial cells in cyst fluid and drainage fluid. A The white arrow shows the endometrial fragments filtered out from cyst fluid. B H&E staining of endometrial fragments; × 200; bar = 50 μm. C IHC staining of cytokeratin 19 in endometrial fragments; × 200; bar = 50 μm. D IHC staining of CD10 in endometrial fragments; × 200; bar = 50 μm. E Negative control for immunohistochemistry staining in endometrial fragments; × 200; bar = 50 μm. F Morphology and identification of primary cultured cells from endometrial fragments. Bright field, × 100, bar = 100 μm; IF field; × 200; bar = 50 μm. Stromal cells (vimentin in green) and epithelial cells (cytokeratin19 in red). G Representative images of tissue in the drainage bag from endometriosis and non-endometriosis patients. H Representative images of H&E staining in tissue from the drainage fluid of endometriosis; × 400; bar = 20 μm. I Representative images of IHC staining of cytokeratin 19 in tissue from the drainage fluid of endometriosis (Negative control was in the box in the upper left corner); × 400; bar = 20 μm

Article Snippet: To determine the epithelial and interstitial structure, the slides were incubated with anti-cytokeratin 19 (10712-1, Proteintech, China) and anti-CD10 (18008-1, Proteintech, China) overnight.

Techniques: Staining, Immunohistochemistry, Negative Control, Cell Culture

Journal: Journal of Ovarian Research

Article Title: The presence of living endometrial cells in ovarian endometriotic cyst fluid may contribute to the recurrence of endometriosis after surgical excision of endometriomas

doi: 10.1186/s13048-022-01018-9

Figure Lengend Snippet: Effect of endometriotic cyst fluid injection on new lesion formation in mice. A The white arrows indicate the new lesions in mice after injection of endometriotic cyst fluid. B Number of mice with or without lesions 2 or 3 weeks after injection. C Representative images of H&E staining of lesions found in mice; × 100; bar = 100 μm. The black frame shows the enlarged field of view; × 400; bar = 20 μm. D Representative images of cytokeratin 19 staining of lesions found in mice (Negative control was in the box in the upper left corner); × 400; bar = 20 μm. E Representative images of CD10 staining of lesions found in mice (Negative control was in the box in the upper left corner); × 400; bar = 20 μm

Article Snippet: To determine the epithelial and interstitial structure, the slides were incubated with anti-cytokeratin 19 (10712-1, Proteintech, China) and anti-CD10 (18008-1, Proteintech, China) overnight.

Techniques: Injection, Staining, Mouse Assay, Negative Control

Journal: International Journal of Molecular Sciences

Article Title: A Low-Sodium Diet Boosts Ang (1–7) Production and NO-cGMP Bioavailability to Reduce Edema and Enhance Survival in Experimental Heart Failure

doi: 10.3390/ijms22084035

Figure Lengend Snippet: Impact of dietary sodium restriction on heart failure plasma biomarkers in mice with DCM. ( A ) Plasma levels of ANP, ( B ) BNP, ( C ) corin, ( D ) cGMP, and ( E ) neprilysin (NEP). All groups were 17 weeks of age. Number of DCM mice n = 6–8 per group. WT mice (dashed line, n = 7–8). Data are presented as mean ± SEM. **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05 (DCM vs. WT), and ++++ p < 0.0001, ++ p < 0.01, + p < 0.05 (DCM on NSD vs. DCM on LSD), ns = non-significant. Data were analyzed by one-way ANOVA with Newman–Keuls multiple comparison test.

Article Snippet: Plasma levels of target proteins were measured by enzyme-linked immunosorbent assay (ELISA) as previously reported [ , , , , , , , ]: ANP (as N terminus-ANP), BNP (as C terminus-BNP) and angiotensin II (Phoenix Pharmaceuticals, Inc., Burlingame, CA, USA); cGMP (Enzo Life Science Inc., Farmingdale, NY, USA); corin (USCN Life Science Inc., Houston, TX, USA); Ang (1–7) (Wuhan Fine Biotech Co., Ltd., Wuhan, China); aldosterone (Abcam Inc., Cambridge, MA, USA) and neprilysin (Boster Biological Technology, Pleasanton, CA, USA).

Techniques: